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1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid

1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid

Product Details:

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Product Description

1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid

  • Synonym: BAPTA, Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid

    • Empirical Formula (Hill Notation): C22H24N2O10

    • Molecular Weight: 476.43

    • CAS Number: 85233-19-8

 assay  


96.0% (HPLC)

fluorescence  

ex 251 nm; em 363 nm (pH 8.0 Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels)

 

ex 274 nm; em 363 nm (With Calcium)

storage temp.  

2-8°C

Description

Application

Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels

1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid was used for the spectrophotometric monitoring of Ca2+ levels. It may be used as chelator of cytosolic Ca²+ to investigate the effect of methoxychlor on cytosolic free Ca²+ concentrations and apoptosis in HA59T human hepatoma cells.[1]

General description

1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid is a highly selective calcium chelating reagent.[4] Effect of microinjected calcium-loaded 1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid (CaBAPTA) on cell-to-cell diffusion of carboxyfluorescein has been studied in staminal hairs of Setcreasea purpurea.[3] It is reported to be a metabolite of 1,2-bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid tetra(acetoxymethyl ester) (BAPTA-AM) in rat urine.[2]



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