2,2-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt
Synonym: Azbts-(Nh4)2, Diammonium 2,2-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonate)
Form : Tablet
2,2-Azino-Bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) Is A Peroxidase Substrate Suitable For Use In Elisa Procedures. This Substrate Produces A Soluble End Product That Is Green In Color And Can Be Read Spectrophotometrically At 405 Nm. The Reaction May Be Stopped With 1% Sodium Dodecyl Sulfate (Sds). Recommended For Elisa (Microwell) Procedures, Not Recommended For Membrane Applications.
Dissolve 1 Tablet In 100 Ml Of 0.05 M Phosphate-Citrate Buffer, Ph 5.0 (Tablets Available As Sigma Product No. P4809). Add 25 L Of Fresh 30% Hydrogen Peroxide
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