HPLC protein standard mixture

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HPLC protein standard mixture Specification

Purity
>98% (main protein components)

Physical Form
Liquid (solution), Lyophilized powder (available variants)

Ingredients
Mixture of purified protein standards (e.g., Thyroglobulin, Gamma globulin, Ovalbumin, Myoglobin, Vitamin B12, and others)

Solubility
Soluble in water and standard HPLC buffers

Taste
Not applicable / NA

Shelf Life
1224 months, depending on storage conditions

Properties
High purity, ready-to-use, accurately quantified protein concentrations

Molecular Weight
Variousrange from ~1,350 Da (Vitamin B12) to 670,000 Da (Thyroglobulin)

Application
HPLC calibration, molecular weight determination, column performance testing

EINECS No
NA (mixture)

Appearance
Clear to slightly opalescent solution or white lyophilized cake

Melting Point
Not applicable (proteins denature, do not melt)

Storage
28C (liquid); 20C (lyophilized)

Ph Level
7.08.0 (in phosphate buffer)

Smell
Odorless

Structural Formula
Not applicable (mixture of proteins with unique structures)

Grade
Analytical grade (HPLCsuitable)

Shape
Homogeneous solution or fine powder

CAS No
NA (Mixture; individual protein standards available with own CAS numbers)

Poisonous
NO

Density
Gram per cubic centimeter(g/cm3)

Product Type
Protein standard mixture for HPLC

Molecular Formula
Mixture (Individual components have unique molecular formulae)

HS Code
3822.00.90

Usage
Preparation of calibration curves, molecular weight mapping, SEC-HPLC validation

HPLC protein standard mixture Trade Information

Main Domestic Market
All India

About HPLC protein standard mixture

HPLC protein standard mixture

analytical standard

Properties

Related Categories	Amino Acid, Peptide & Protein Standards, Analytical Standards, Analytical/Chromatography, HPLC Protein and Peptide Standards, Molecular Biology, Protein Chromatography, Proteins / Enzymes Standards, Proteomics
grade	analytical standard
application(s)	HPLC: suitable
	gas chromatography (GC): suitable
format	multi-component solution
storage temp.	20C

Extensive Molecular Weight Range

By spanning approximately 1.3 kDa to 670 kDa, this protein standard mixture ensures accurate calibration across a broad spectrum of HPLC and SEC-HPLC applications. This allows users to characterize columns and verify molecular weight determinations with confidence, regardless of their analytical needs.

Flexible Product Formats

The mixture is available as pre-aliquoted liquid vials or lyophilized powder kits, offering flexibility in storage and handling. Lyophilized kits can be conveniently reconstituted with sterile distilled water or buffer to suit laboratory protocols. Tamper-evident packaging ensures sample integrity throughout shipping and storage.

FAQs of HPLC protein standard mixture:

Q: How should I reconstitute the lyophilized protein standard mixture?

A: To reconstitute the lyophilized protein standard mixture, use sterile distilled water or an appropriate buffer such as phosphate or Tris buffer, as specified in the product instructions. Ensure thorough mixing to achieve a homogeneous solution for accurate results.

Q: What is the recommended storage condition for the standard mixture?

A: Liquid formulations should be stored at 28C, while lyophilized variants are best kept at 20C. Proper storage within these temperature ranges maximizes shelf life, which is typically 1224 months based on format and storage environment.

Q: When can I use this product for HPLC calibration and performance testing?

A: The protein standard mixture is suitable at any stage of HPLC calibration, column performance testing, or molecular weight determination. It offers reliable standard curve generation for SEC-HPLC and other routine validation processes.

Q: Where are the component proteins traceable to international standards sourced from?

A: Each component protein in the mixture is referenced to internationally recognized standards, ensuring high traceability and reliability. This provides consistency and comparability for analytical laboratories worldwide, including suppliers in India.

Q: What are the main benefits of this protein standard mixture for laboratory applications?

A: Key benefits include high purity (>98%), accurate protein quantification, compatibility with common UV detection wavelengths (214 nm, 280 nm), and ready-to-use formats. This enhances analytical confidence and workflow efficiency in HPLC-related tasks.

Q: Is the product compatible with various buffers and detection methods?

A: Yes, the standard mixture is soluble in water and standard HPLC buffers, such as phosphate and Tris buffer. It is designed to perform well with UV detection at 214 nm and 280 nm, making it versatile for most protein analysis workflows.

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