About Human DNA for whole-genome variant assessment (Daughter of Utah/European Ancestry)
Human DNA for whole-genome variant assessment (Daughter of Utah/European Ancestry)
Properties
| Related Categories | Analytical Standards, Analytical/Chromatography, NIST Standards, Pharmacopeia & Metrological Institutes Standards |
Stringent Quality for Precision GenomicsOur DNA is molecular biology grade, with purity exceeding 98% (A260/A280 ratio), and meticulous verification through gel electrophoresis and STR profiling guarantees high genomic integrity. The absence of RNase, DNase, and protein contamination ensures reliability for downstream applications such as sequencing and genotyping.
Optimized for Advanced Research ApplicationsIdeal for whole-genome variant assessment, SNP genotyping, NGS, and array CGH, this DNA serves as a robust reference material for assay validation. Its high molecular weight and integrity support comprehensive genomic studies with consistent results.
FAQs of Human DNA for whole-genome variant assessment (Daughter of Utah/European Ancestry):
Q: How is the genomic integrity of this human DNA verified?
A: Genomic integrity is confirmed by gel electrophoresis, which assesses the DNAs high molecular weight and absence of degradation, as well as by STR profiling, ensuring accurate identification and quality.
Q: What are the recommended storage conditions for this DNA sample?
A: Store the DNA at 20C or lower in a tightly sealed container and avoid repeated freeze-thaw cycles to maintain its integrity and extend shelf life, which is up to 24 months under proper storage.
Q: When should I use this DNA sample in my research workflow?
A: This DNA is best utilized in steps requiring reference-grade material for whole-genome variant assessment, SNP genotyping, next-generation sequencing, array CGH, and validation assays, especially when high purity and integrity are critical.
Q: Where does this DNA originate from and how is cross-reactivity avoided?
A: The DNA is derived from a daughter of Utah/European ancestry (NA12878, Homo sapiens). No cross-reactivity with non-human genomic DNA has been detected, supporting its use in human-specific genomic analyses.
Q: What process should I follow to reconstitute this lyophilized DNA?
A: To reconstitute, dissolve the lyophilized powder in nuclease-free water or TE buffer to achieve the desired working concentration, typically 100 ng/L. Ensure gentle mixing to maintain molecular integrity.
Q: How is the absence of contaminants such as RNase, DNase, and proteins assured?
A: The DNA undergoes rigorous purification and testing processes to confirm it is free from contaminating RNases, DNases, and proteins, making it suitable for sensitive molecular biology protocols.
Q: What benefits does this DNA offer for sequencing and genotyping applications?
A: Its high molecular weight, double-stranded nature, and molecular biology grade purity enable reproducible sequencing and genotyping results, providing robust reference material for assay validation and research reliability.