About Human immunoglobulin (molecular size) BRP
Human immunoglobulin (molecular size) BRP
European Pharmacopoeia (EP) Reference Standard
Synonym: -Globulins human
CAS Number 9007-83-4
Properties
| Related Categories | Analytical Standards, Analytical/Chromatography, EP Standards, EP Standards G - H, Pharmacopeia & Metrological Institutes Standards |
| form | neat |
| format | neat |
Reliable Calibration and StandardizationHuman immunoglobulin (molecular size) BRP serves as a robust molecular size standard for accurate calibration in protein analysis, including gel electrophoresis and quality control. Its high purity and precise molecular size (~150 kDa IgG) ensure reproducible results, supporting compliance with stringent pharmaceutical and analytical requirements.
Convenient and Stable FormatThe product is provided as a lyophilized (freeze-dried) solid in a tightly sealed glass vial. This formulation, augmented with buffer salts and stabilizers, maintains protein integrity and prolongs shelf life. Stability is assured when stored refrigerated at 28C, with a typical shelf life of 23 years as per the expiration date.
Ease of Use and ApplicationDesigned for intuitive use, simply reconstitute the powder with distilled water to achieve a clear, neutral pH (7.0) solution. Its solubility in water or saline streamlines preparation, making it suitable for a range of calibration and reference tasks in molecular biology and therapeutic protein analysis laboratories.
FAQs of Human immunoglobulin (molecular size) BRP:
Q: How should I prepare Human immunoglobulin (molecular size) BRP for laboratory use?
A: To prepare this product for use, reconstitute the lyophilized powder by adding distilled water as recommended in the instructions. Gently mix until fully dissolved. It is then ready for calibration and quality control applications.
Q: What is the main benefit of using this standard in gel electrophoresis?
A: This reference standard provides a precise molecular size (~150 kDa) for accurate calibration during gel electrophoresis, supporting reliable molecular weight determination and ensuring consistent quality control in protein assays.
Q: What are the recommended storage conditions for this product?
A: Store the sealed vial at 28C under refrigeration to maintain stability. Always keep the vial tightly closed, and avoid repeated freeze-thaw cycles to preserve product integrity.
Q: When should I use this standard during protein analysis?
A: Use Human immunoglobulin (molecular size) BRP as a size marker during protein gel electrophoresis, or for the calibration and standardization of instruments used in protein size determination and therapeutic protein analysis.
Q: Where is this product typically applied?
A: This standard is commonly used in laboratories for pharmaceutical analysis, research, and quality control, particularly in settings that require compliance with European Pharmacopoeia (Ph. Eur.) standards.
Q: What is the process for reconstituting the powder?
A: Add the specified volume of distilled water directly to the vial. Allow the powder to dissolve completely by gentle swirling or inversiondo not shake vigorously. Use immediately after reconstitution for best results.