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3,3′,5,5′-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine
3,3′,5,5′-Tetramethylbenzidine
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Product Code : 1300
Product Description
3,3′,5,5′-Tetramethylbenzidine
Standard For Gc


Synonym: Bm Blue, Sure Blue Tmb, Tmb Blotting Plus, Tmb Substrate, Tmb
  •     Cas Number 54827-17-7
  •     Linear Formula [-C6H2(Ch3)2-4-Nh2]2
  •     Molecular Weight 240.34
Properties 
  • Grade   :    Analytical Standard
  •       Standard For Gc
  • Assay    :   ≥99.0% (Gc)
  • Shelf Life  :     Limited Shelf Life, Expiry Date On The Label
  • Mp     :  166-171 °C
  •       168-171 °C(Lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (Tmb) Is A Noncarcinogenic Substitute (Ames Test Negative) For Benzidine[] Suitable As Peroxidase Substrate For Use In Elisa Procedures. The Substrate Produces A Soluble End Product That Is Pale Blue In Color And Can Be Read Spectrophotometrically At 370 Or 620-650 Nm. The Tmb Reaction May Be Stopped With 2 M H2So4 (Resulting In A Yellow Color), And Read At 450 Nm. A Sensitive And Specific Reagent For The Detection Of Blood,[] Assay Of Hemoglobin,[][][] Assay Of Peroxidases.[][][]

Other Notes

Standard For Determining Aromatic Amines Released From Azo-Dyes In Commodities[1]


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