3,3′,5,5′-Tetramethylbenzidine (Tmb) Is A Noncarcinogenic Substitute (Ames Test Negative) For Benzidine Suitable As Peroxidase Substrate For Use In Elisa Procedures. The Substrate Produces A Soluble End Product That Is Pale Blue In Color And Can Be Read Spectrophotometrically At 370 Or 620-650 Nm. The Tmb Reaction May Be Stopped With 2 M H2So4 (Resulting In A Yellow Color), And Read At 450 Nm. A Sensitive And Specific Reagent For The Detection Of Blood, Assay Of Hemoglobin, Assay Of Peroxidases.
Standard For Determining Aromatic Amines Released From Azo-Dyes In Commodities
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